Generation of Human Neutrophils from Induced Pluripotent Stem Cells in Chemically Defined Conditions Using ETV2 Modified mRNA

Summary: This protocol describes a rapid and efficient feeder-, serum-, and Western Bridles xeno-free method for neutrophil generation from hiPSCs using ETV2 modified mRNA (mmRNA), which directs hematoendothelial programming of hiPSCs.Hematoendothelial progenitors were cultured with GM-CSF, FGF-2, and UM171 to expand myelomonocytic progenitors, fol

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Processing Government Data: ZIP Codes, Python, and OpenRefine

While there is a vast amount scissor of useful US government data on the web, some of it is in a raw state that is not readily accessible to the average user.Data librarians can improve accessibility and usability for their patrons by processing data to create subsets of local interest and by appending geographic identifiers to help users select an

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